Document Details
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Document Type |
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Thesis |
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Document Title |
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Effect of differnt photoperiods on the mice ovary growth and ovum development. تأثير اختلاف الفترات الضوئية على نمو المبايض وتكوين البويضات في اناث الفئران |
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Subject |
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Biological |
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Document Language |
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Arabic |
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Abstract |
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The aim of the present research is to study the effect of different photoperiods on ovary growth an ovum development of virgin female mice Mf1, Mus mussulus. The animals were obtained from King Fahad Center for Medical Research, King Abdul Aziz University in Jeddah. The animals were divided into five groups after weaning and exposed to different photoperiods: balanced photoperiods 12L:12D; continuous light (C.L.); continuous dark(C.D.); Long photoperiod (16L); short photoperiod (8L). The experiment was last for three months, during which the body and ovary weight was determined and blood samples were taken to measure the Progesterone and estrogen at three intervals (30, 60 ,90days). In addition, samples of the ovary tissues were taken for histological study and estimate the numbers of developing and mature follicles and the corpus luteum as well as the different ovarian follicles structures at the same intervals. The body weight of the 30 days old animals showed significant decrease the C.L. group, but no significant differences were observed in the animals of the 60 day of age, while a significant increase in the body weight were noticed in C.L animals group of 90 days when compared with the control. There was no significant differences in ovary weight at age 30 days in all groups, however, a significant decrease in the ovary weight were obtained at the 60 and 90 days of animals age of the C.D. group as well as a significant increase in that of the C.L. group when compared to that of the control. There was a significant increase in the progesterone concentration in the blood of C.L group and a significant decrease in both C.D. and 8L animal groups of the 30days of age , while that 60 and 90 days age showed no significant differences when compared with the control. There was a significant reduction in the estrogen concentration in the animals blood of C.D., 16L and 8L groups of 30 days of age. There was no significant differences in the estrogen concentration in the blood of all groups at 60 days of age, however, there was a significant reduction in estrogen concentration in C.D. group of 90 day age compared with that of control group. A part of 8L group, there was a significant reduction in developing follicle numbers of all experimental groups of 30 days of animal age compared with control group. However, there was no significant differences in developing follicles numbers of the 60 and 90 days of animals age, while there was a significant increase in the number of mature follicles at 30 days of animal age for the 16L group and no significant differences in all groups of the 60 days of animals age. There was a significant reduction in the number of mature follicles at 90 days of animals age for groups C.D., 16L and 8L compared with that of control. The corpus luteum number showed no significant differences between all groups of the 30 days of animals age, while at 60 days of animals age there was a significant increase in the corpus luteum number of C.D. and 16L groups compared with that of the control group. However, a significant reduction in the number of corpus luteum in all experimental groups of the 90 days of animals age. Histological examination by light microscope of ovary sections of the animals of the 30 days age revealed an increase in the egg size with clear nucleus and nucleolus in the C.L. group, while that of C.D. group showed a reduction in the eggs sizes compared with control group. There were a rupture of some developing follicles in 16L group and shrinkage of egg sizes and rupture of the granulose cells in 8L group. An increase in egg sizes were noticed in all groups of the 60 days age specially in C.L. group. A cleared zona pellucida around the eggs in the C.L. group with disarrangement of the corona radiate and a reduction with degeneration of zona pellucida in the C.D. group. There were an increase in egg sizes in group C.L. of the 90 days of animal ages, while that of 16L group have a large nuclei with clear nucleolus compared the that of control group. However, the eggs in group C.D. of the 90 days of animal age were degenerated and reduced in size, whereas that of 8L group contained developed follicles which have an egg with large nucleus and clear nucleolus and presence of few degenerated follicles. Corpus luteum were very rare in all groups of the 30 days of animal ages, while the number of that of the animals at 60 days were large in size in C.L., 16L and 8L groups compared with that control. On the other hand the corpus luteum of the 90 days of age were large in size in C.L. and 16L group, while they were small in size and shrinking in the 8L group. There were some changes in the size and number of the interstitial cells of the ovary in all experimental groups. The results of the present study suggest that the change in photoperiod has a very sharp affect on the normal development and egg formation of mice ovary. Continual exposure to light as well as deprivation of it lead to severe damage to ovary structure and function. It can be concluded that the balance in the photoperiod of animals is important for normal reproductive activity of female mice. The results of the present study pave the way for future research on the effect of photoperiods on the fertility on reproduction of various animals. |
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Supervisor |
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Prof. Ali Ahmed Salem Alrobai |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1430 AH
2009 AD |
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Added Date |
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Sunday, December 5, 2010 |
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Researchers
| تركي عمر عصر | ASAR, TURKY OMAR | Researcher | Master | |
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