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Faculty of Science and Arts - Khulais
Document Details
Document Type
:
Article In Journal
Document Title
:
Purification and Characterization of two glycoproteins from the cattle tick Boophilus annulatus
تنقية وتعريف نوعين من الجليكوبروتينات من يرقات قراد البقر من نوع بؤفيلس أنيولاتس
Subject
:
Vaccine production
Document Language
:
English
Abstract
:
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation
ISSN
:
1229-845X
Journal Name
:
Journal of veterinary sceince
Volume
:
8
Issue Number
:
2
Publishing Year
:
1428 AH
2007 AD
Article Type
:
Article
Added Date
:
Saturday, January 15, 2011
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
عمرو السيد الحكيم
El Hakim, Amr El Sayed
Researcher
Doctorate
aselhakim@hotmail.com
ياسر عزت شاهين
Shahein, Yasser Ezzat
Researcher
Doctorate
أميرة كمال أبو العلا
Abouellela, Ameera Kamal
Researcher
Doctorate
Files
File Name
Type
Description
28633.pdf
pdf
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