Document Details Document Type : Article In Journal  Document Title : Apoptosis study on the effect of PMF on different cancer cells Apoptosis study on the effect of PMF on different cancer cells   Subject : Biological  Document Language : English  Abstract : PM 701, a natural liquid product, has anticancer activity. The bio-active fraction G, which is coded PMF has recently been isolated from PM 701. PMF was proved to have cytotoxic properties against cancer cells. The target of this study was to investigate the apoptotic effect of PMF in the human cancer cell lines. PMF at concentration of 60 and 100 μg mL-1 were incubated with cells during 48 h culture. Metabolic activity were measured colorimetrically by MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide). Apoptotic cell death was also determined by the TUNEL method (terminal deoxynucleotidyl transferase (TdT)-biotin nick end-labeling). The results showed that PMF induced apoptosis in association with increased number of TUNEL positive cells. MTT results showed that PMF decrease cell proliferation via inhibiting metabolic cell activities. We conclude that PMF has anti-cancer effects by increasing apoptosis and altering cellular metabolic activity.  ISSN : 1819-155X  Journal Name : International Journal of Biological Chemistry  Volume : 5  Issue Number : 2  Publishing Year : 1432 AH 2011 AD  Article Type : Article  Added Date : Tuesday, June 12, 2012  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email فاتن خورشيد Khorshid, Faten Researcher Doctorate fatenkhorshid@yahoo.com سوسن عبدالعزيز رحيم الدين Rahimaldeen, Sawsan A Researcher Doctorate srahimaldeen@kau.edu.sa J S AL-Amri AL-Amri, J S Researcher Doctorate   Files File Name Type Description  33580.pdf pdf Abstract Back To Researches Page