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Faculty of Sciences
Document Details
Document Type
:
Article In Journal
Document Title
:
Development of Mediator-Free Acetylcholine Biosensor Based on Acetylcholine Oxidase Immobilized Micro-Chips
Development of Mediator-Free Acetylcholine Biosensor Based on Acetylcholine Oxidase Immobilized Micro-Chips
Document Language
:
English
Abstract
:
Acetylcholine (ACh) biosensor is developed based on mediator-free acetylcholine oxidase (AChOx) by self-assembled monolayer (SAM) onto lab-made micro-chip. The simple cyclic voltammetry (CV) method is utilized in mediator-free system in phosphate buffer solution (PBS, 0.1M) at room conditions. The analytical parameters of AChOx fabricated electrode employed a lower detection limit (DL, 0.136 nM), a wide linear dynamic range (LDR, 1.0 nM to 1.0 mM), good linearity (R= 0.9991), and higher sensitivity (2.7329 mu AmM-1 cm(-2)) where a tiny sample volume (70.0 mu L) is required. The micro-chip system executes a simple and efficient approach to immobilize the enzymes onto SAM modified surface, which can improve the biosensor performances for a large group of biomolecules in broad scale of biomedical applications in health-care fields.
ISSN
:
1570-1646
Journal Name
:
CURRENT PROTEOMICS
Volume
:
9
Issue Number
:
4
Publishing Year
:
1433 AH
2012 AD
Article Type
:
Article
Added Date
:
Sunday, July 9, 2017
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
M Rahman
Rahman, M
Researcher
Files
File Name
Type
Description
42055.pdf
pdf
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